FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564807
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCTGTTGACATCTCGTATGCCGT6650.11773933396717817TruSeq Adapter, Index 15 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2600.044.1082769
CACACGT3050.039.9505277
ACACGTC3150.036.406838
AGCACAC4250.028.670385
GCACACG4100.027.97116
AGAGCAC4400.027.695433
GAGCACA4700.025.9276374
GAAGAGC7653.6379788E-1215.966151
AAGAGCA8351.2732926E-1114.599172
CAGTCAC3053.092282E-119.87013120-24
CCAGTCA3752.1827873E-118.7922520-24
AGTCACC2951.7234925E-88.74689520-24
CTCGTAT3401.8983701E-77.590024535-39
ACGTCTG3102.9706462E-67.398807510-14
CTCCAGT4506.1962055E-86.68975515-19
CGTCTGA4002.8102531E-66.45083510-14
GTCACCT4251.1090651E-66.40867320-24
ACTCCAG4251.1090651E-66.40867315-19
TCTCGTA3451.0365983E-46.23335335-39
GTTGACA4903.0879164E-76.14441430-34
Produced by FastQC (version 0.11.5)