FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594846
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAT4900.00869649110.5134581
CTCTCGT5950.00322476759.895021
CTCGGTT7250.0015909779.1358341
CTTAAAG15101.6354534E-88.77281951
CTCCAAC12205.20874E-68.4452371
CGACAGA9853.296039E-48.2186431
CCCAAAT11752.9706378E-58.1423381
CTTCGAT16608.157258E-87.98009441
CTCAGAA9400.00188581817.82917071
CTCCCAA11301.6731689E-47.81531431
CTCAGAT8500.00669908347.7923281
CTTAAAT14305.2100586E-67.7196721
CTCAAGG12556.754873E-57.62330441
CTCCAAT14606.991566E-67.56104851
CTTCGAG11852.8971926E-47.4525781
CCTTCAT21051.0406438E-87.34194041
CTCTACA10250.00440064967.17992261
CTCGAAG11300.00143027037.1640381
CTTGTAA17802.085124E-67.0286611
CTATAAC10900.0079881946.7517621
Produced by FastQC (version 0.11.5)