FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695016
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAA1900.00599859919.0165255
CTCAAAT6009.250107E-814.6379681
CTCAATC4850.00838767610.5634821
CTATTGT4900.00905025910.4556911
CTTCGAT7903.5369943E-49.2645381
CTTCAAC8356.1519735E-48.7652511
CTTTGAA11853.7770205E-68.6469021
CAATCAT8050.004836028.0790694
CCCTGCT8250.00537683167.98434731
CTTCTTT21101.7826096E-107.978041
CAATGAA8550.0074067377.70419451
CCGTTTT13252.0000343E-57.6353143
CTTCTGT8750.0091068617.52809861
CTCCTTT12053.7328596E-47.28861571
CTTTTGT11100.00125214247.2530481
CCTTCTT12304.7242452E-47.1404731
CAAAAGA13401.6213884E-47.10050771
CTTTTAT14555.8257774E-57.04232171
ATATTCC13050.0078132356.0911176
CTTTCTT18800.00158779085.45030741
Produced by FastQC (version 0.11.5)