FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12932744
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCT28653.1577656E-96.31165171
CTTCAAT161150.06.149191
CTCAAAT94950.06.01810461
CTTCGAA80550.05.92660571
CCCACGT50150.05.91344451
CTCCAAT96700.05.9091941
CTCTGAT72600.05.8781821
CTTAAAT100000.05.85885331
CTTCGAT144600.05.8025291
CCCAAAT82550.05.78301671
CCCAACT55550.05.729231
CTCTAAT40403.8198777E-115.72922951
CTTAAAC56600.05.62294531
CTTGAAT119250.05.58029371
CTTTAAA144200.05.5678221
CTCAAAA163250.05.53840161
CTTAGAC20902.0769192E-45.53734161
CTTTAAT145350.05.523771
CTTAAAA157150.05.477221
CTCCAGT78050.05.46772581
Produced by FastQC (version 0.11.5)