FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1419414
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAG6850.00118162759.4383926
GTCCGAC6950.00128184889.3546551
TCGAATT9001.6073162E-48.782822
CCTTCAT20303.1477975E-66.4054041
ATATAAC13900.00234162626.2017256
CTTTGAT22852.1381618E-55.6905781
CTTCCGT19254.1594438E-45.62899161
CAACAAG16800.00293004145.5899011
CTTTCAT23453.235697E-55.54497671
CTGATGC17250.0040052765.4440781
CTTTTAC19300.00261035865.24011471
CCCGCCG18150.0072772215.17412331
TTGTTGC18250.0082815665.1171316