FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043643
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGA1400.001476310938.700764145
ACACGTC4653.96187E-614.3889538
CACACGT4454.1755735E-513.5320837
CACGTCT4709.997785E-411.3887039
CTCGAAC4950.001472158110.8734261
GGGGGGG1850.00342959479.822215140-144
GCACACG7153.2025407E-49.3578516
AGCACAC8200.00125351058.159595
AGAGCAC9304.943667E-47.9139243
ACTTTGT7950.00862767.5745626
TAGATAT8950.0029649767.47582533
GAGCACA10150.0012571477.25118164
TAATGGT10000.0087124486.69086369
CTTCGCC14750.00144922475.9297031
CTTCGGC15300.00224365955.7165441