FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257759
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACT3850.001858338112.9260491
CCCAAGT6053.190226E-511.7509531
CTCCGAG5300.001643578610.7310591
CTCACAG6200.00602215539.1733261
CTCATTC11654.5306479E-79.1536391
CTCTATT11052.1803953E-69.0072911
CCCAAAG13906.999835E-88.694861
CTCAGAA7400.00262302358.6464791
CCTCAAT9352.727352E-48.3639141
ATTCGCT8550.00104035838.31500151
CACCAGG8600.00110220688.2666591
CCCAGAT11553.691123E-58.0018391
CTCAAAA18201.0422809E-87.8124471
CTTCAAA27400.07.7839341
GTCTTGT9150.0020318497.76975631
CTTCGAT12106.59022E-57.6381191
CAACGAA11603.372862E-47.35447551
CTCAAAT9700.00359952967.32920271
TCTTATG11000.00162630887.07738977
CGAAAGG10200.0058700826.9699281
Produced by FastQC (version 0.11.5)