FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753538
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTAA2104.4560802E-420.6141725
CCCCCAT3355.673113E-415.1221951
ACAGTCC3250.00824857513.3199273
CCCAGCT5801.7227516E-512.4776741
ACAACTT6403.5735193E-612.4007133
CTCAAGA5350.001530972210.8217581
GTACCTT4800.00863417810.5225158
CTCCACG8400.00699324867.75398251
CAATGAA9450.00233916617.65825461
CTTCAAA14053.327694E-46.69620371
CAATGCC11900.00294086386.6897111
ATATTGT12250.0041109876.479176
CAAAGAA18402.1923016E-45.899771
CAAAAGA16100.00172433615.8435811
CTTTATT14900.00467493665.8284971
TTTTGCA19600.00320523125.15388547
Produced by FastQC (version 0.11.5)