FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539232
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTGGAGAGTATCTCGTATGCCGT7040.1305560500860483TruSeq Adapter, Index 10 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2850.050.445427
CACGTCT2750.047.051829
ACACGTC3450.043.755928
GAGCACA3750.034.504674
AGAGCAC3800.034.04753
AGCACAC4050.031.9487655
GCACACG5250.024.6461936
GAAGAGC6000.022.799441
AAGAGCA7450.019.2961222
TACGTCC2500.001474065617.2523359
GAGAGTA2901.8189894E-1210.90563730-34
ACGTCTG3001.8189894E-1210.54231110-14
AGTCACT2703.3651304E-1010.11617220-24
GGTAAAG6000.0041350419.5997651
CAGTCAC3155.4533302E-98.67100420-24
TCACTGG3751.9645086E-108.43369225-29
GGAGAGT3259.540599E-98.40420430-34
GTCACTG3504.105459E-98.21463520-24
CTCGTAT3701.3442332E-98.1591335-39
CGTCTGA3401.8133142E-77.61075910-14
Produced by FastQC (version 0.11.5)