FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779672
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG15704.1477688E-77.7833671
CCTTAAT10000.00435243737.1881681
CTCAGCG10050.00456896567.1524061
CACTGTT12250.0042848156.4546821
CTTGGAT12750.0064988876.20155671
CTAAAAT17005.573016E-45.9196681
CTACAAG15050.0056443315.731431
CTCATAA17250.0042481865.417171
CTCAAAA22054.8492113E-45.2159041
CTGAAAT27005.957613E-55.05834051
Produced by FastQC (version 0.11.5)