FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590537
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGGA7300.00205736378.8813491
GTTCGAT14459.571013E-77.9764821
CGAAACT10458.248025E-47.5447633
CTCGGAA9750.00332840567.3884721
CTAAAAG11150.00155700747.10684821
CTTTGAT23451.7294951E-86.7583251
CCAAAAT16354.612225E-56.6089541
CTTTAAT20152.9196544E-66.43512061
CCCGAAT14957.498214E-46.26413971
CTTCCGA18453.6239988E-56.24716331
CTCCAGA14000.00245993056.17465071
CTTCAGA14200.00288356936.08768461
CCCGGAT20953.633266E-55.84553341
CTTCGGT16200.00196604525.78079461
AATTTTG16150.00201309685.7693445
CAATTCA16200.0020878565.7517174
CTTTCAT26256.2010276E-65.4885791
AGGAATC17150.0041027185.43310934
TAAATAT34352.0098742E-75.2165393
ATTAAAT19750.00360572925.10646251