FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694896
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA6707.6914113E-49.8915661
CTTGGTT9803.148338E-59.0168031
CTCCAAA17904.3655746E-108.6390041
CCCACTT7950.00365145328.3362891
CTCAAAT15802.6386497E-67.4569341
CTTCCCT13501.6532268E-47.0909921
CCCAGAT14957.704529E-56.8957941
CCCAAAT11100.0094666686.6339841
CAACAAT23801.0755684E-76.49740171
CCTCTGT14805.0865597E-46.46813441
CTCCGCT12750.0050561016.35301541
CAACGAT16352.4728046E-46.30532841
CTTGAAT13550.0095038565.97792961
CTCCAAT15200.004820685.8134651
TCAAATG19153.8540395E-45.6610272
CTTAAAT15800.0073995675.59270051
CTTCAAA30703.7000427E-75.51679561
TCGCTTT18800.00186861765.3816733
CTTTTGG21903.1260724E-45.37988851
CATCAAT31754.0669474E-65.10242181
Produced by FastQC (version 0.11.5)