FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590434
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCG500.00458240158.326263145
GTCTAAT2104.2993773E-420.722731
CTCAAGG4951.8532228E-716.117681
CTTCTAA3200.007188210313.5992921
CCCAGAA5250.001283422311.0521221
CCTATCA4650.006560567310.9184281
ATCAGAC5450.001753111710.6465411
CTTGGAA7702.627019E-510.3613641
ATATTAC7350.0021345048.8435616
CTTCTGG7400.00218420698.8211621
CTAAAAA6700.009641418.6602461
TCAAGGA10256.665121E-58.4560232
CTCCTTC8450.00722666137.72504141
CTTTCAT9500.00240908457.634691
CTTCAAA10400.00582602156.97399571
CAACAAG10500.0063923246.90757661
CTTTCAG11600.00219485826.8778031
CTCTTTT12050.00327453586.6209551
TTTTTGC15750.00135821695.960717
Produced by FastQC (version 0.11.5)