FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357033
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4551.0182521E-716.9920587
CACGTCT5402.2864697E-916.9205429
ACACGTC5508.752413E-714.0570658
CTAATGG3250.009808077512.9762323
GAGCACA8656.7939254E-912.1891054
GCACACG8356.918108E-710.9426266
AGCACAC8559.385021E-710.6866585
AGAGCAC10309.723234E-58.1888833
GTCAAAT8100.0064105667.83054451
CTCAAAA13654.1449268E-57.2281941
GTTCAAG13603.0984174E-46.73657131
TCACCTG12100.00476098676.38957558
ATTCCCA12700.00787182156.0877066
ATTTCCC16254.1642564E-46.0553516
GGATAAG15100.00113740096.0510556
CAAAAAA28251.0129788E-65.48831031
CAGGAAT20200.00110665565.23328451
GAAGATC19100.00322517055.15218354
Produced by FastQC (version 0.11.5)