FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492128
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG15251.8189894E-1210.4359691
CTCACGA6450.0072246178.9724341
TAGGTTT7250.00196014658.9287063
CTCAGAT7400.00224082088.7981391
CTAAAAT13553.382429E-78.5420221
CTTCAAT21002.3646862E-118.2674571
CTCCGAT12559.587646E-68.0698281
CACAGAG9850.00352939157.34418731
CAACCAT10850.00111504527.33403441
CTCATTT13903.7315538E-57.2860681
CTTAAAG12054.2780646E-47.20400861
CTATGAT14657.453779E-56.91306161
CTTCGAT17802.7306996E-66.90890031
TTCAATT21101.2704004E-76.82011462
CTTTCGT13802.691787E-46.8146611
CTCAGAA10650.00753653146.7925121
CCATTGA16354.71205E-56.59868674
CCTCAAA14404.5238144E-46.5307171
CTTTACT12200.0038404786.52248141
CTTCTAT15751.9172046E-46.4302441
Produced by FastQC (version 0.11.5)