FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070745
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT4904.876982E-513.3103031
CTCAACT4700.007155292710.7929871
CCCATAT9703.1940508E-69.7120981
CTCCGTA8801.1481013E-49.05841
CTCGTTT7300.00194804788.9343121
CTCATAC6500.00758420778.919041
CTAATAT7350.00207273368.8735351
CTCCCTT7600.0028074068.5816411
CATCGAT8457.653332E-48.5761
CAACCAG9502.6330297E-48.3909391
CTCATAT9000.00142936998.0519111
CTCTCTT9000.00142936998.0519111
CCCAGAT8200.00557085557.9537171
CTTCGAA9250.00187252667.8342921
CAAACAT12105.191329E-57.7857321
CCTTGAT15854.231788E-77.7725071
CATGAAT10306.27979E-47.73921541
GACAATC10306.4745435E-47.7171572
CACAGGA9750.0031384427.43253371
TCGATTT11803.4157585E-47.34613853
Produced by FastQC (version 0.11.5)