FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242966
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCACT3650.001028552513.980941
CCCAATT5109.6632325E-411.435391
CTCAGAG5200.001136834511.215481
CTCGAAA8555.858201E-711.0843041
CTACACA4650.00632292110.9742861
CTACAAT9351.8509108E-610.1359151
CTCACTT6950.00117913269.4403681
CCCAAAG8457.214698E-48.6272921
CCTGACA8508.5413747E-48.4823
CTCAGCG7850.0035652258.3580321
CTCAAAT13653.0740575E-68.0110571
CTTTAAC11151.5984963E-47.8458071
CTCTCCC8750.009444937.4983491
CCAAGCT11000.00118819447.29006241
CTTCTAT14154.2620843E-57.2127821
CTGGAAT15551.9389767E-57.0322141
CTTATAA13902.6742258E-46.8180431
CAATTAT16203.4355002E-56.75005671
CTTTGGA14153.3251455E-46.6975831
CTTCGCC16454.251175E-56.6474731
Produced by FastQC (version 0.11.5)