FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294149
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4502.1457272E-615.2649197
ACACGTC5206.01789E-714.5310288
CACGTCT6104.9278206E-511.2610059
GCACACG6951.850433E-49.8829966
AGCACAC7554.2495748E-49.0975935
GAGCACA7350.00336905038.4106324
AGAGCAC7850.0061039657.87340163
CTTCGAT10300.00108039687.3556681
GTGAATA11750.00444479436.4317472
CAAAACA16350.00373447875.47636371
GTTTGAT18906.17445E-45.4663341
Produced by FastQC (version 0.11.5)