FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846684
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAC2650.002133007616.3266779
CTCACTG4705.163943E-412.3205221
CTTCACT5650.002407754510.248931
CTACTAT6456.343699E-410.0999631
CTTCCGA8951.3176865E-59.7049921
CTTCTAT11753.916821E-68.6243661
CCTGTAC7950.0042589238.194311
CTCCAGC13452.3895638E-57.53429751
GTTCCTT11652.8782454E-47.45576671
CTTCCGT10400.00594774736.9599111
CCCCCCC2400.00333950656.610817445-49
GTTTTAT14404.4836887E-46.5345831
CTTTCCG15852.0612957E-46.39345741
CTTCATT13600.00168004396.3867411
CTTTGAT14756.001308E-46.37952471
CTTCAAT12800.00628775736.220421
TATCTGC13150.0086377886.03268535
TTCATCC13200.0089668266.0108993
AGAAAGC15050.00521682955.77140761
CAAAGAT15900.009567785.46287351