FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2697559
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAT4450.005065202711.3068991
CTCCAAT20700.09.3755971
CTTAGAT6450.00761663638.9152971
CTTAATT13905.5036435E-78.2739081
CTCACTT8900.00138892148.0763571
CTTCGAG24251.8189894E-128.0030861
TCCGAGT10801.3116976E-47.98158452
CTCAAAA33950.07.8337091
CTTTGAT36550.07.6697771
CTTCCGA25655.456968E-127.5662711
CTTCCCT17252.3155735E-77.50047731
CTTGAAT25002.1827873E-117.4754761
CTACGAT18358.22165E-87.44257161
CTTACGA12609.514452E-57.41614681
CTAAGAA9700.00323574627.41026541
CTCAAAT20751.2460077E-87.274561
CTTGGAT14851.2404893E-57.26056341
CCTCGAT28801.8189894E-127.2378741
CTTTAAC16951.4006073E-67.20916031
CTTAAAC12054.6072237E-47.15813161
Produced by FastQC (version 0.11.5)