FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113929
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3300.00944675413.0499557
ACACGTC5852.050091E-512.269198
CACGTCT4500.005561611.1649639
AGCACAC5900.00369648179.7326075
GAGCACA6150.0051982259.338654
CCTTGGG7050.00149269469.1994861
AGAGCAC7100.00164777899.100253
CTTTTGG10700.00100115477.4083091
CTTCTGT10050.00445535737.17041251
CTTTTAT15452.0819829E-56.99637371
CTTGATT11700.00257656686.77512031
ATCCGGT12150.00382785316.524191
CCCGACG13800.00208322176.2663171
CTTCGAT14957.4601575E-46.26631641
CCTCGAT15259.480865E-46.14304541
GTTTATT13300.009794945.9600681
CTTTGAA14600.00391395585.9229571
Produced by FastQC (version 0.11.5)