FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193056
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACCACCACTAATCTCGTATGCCGT11940.10007912453396992TruSeq Adapter, Index 7 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5050.040.0533837
CACGTCT5350.037.8074049
ACACGTC5450.037.113698
GCACACG6450.031.3596296
GAGCACA8300.024.3708534
AGCACAC8450.023.937235
AGAGCAC8600.023.5177483
GAAGAGC15250.013.29031851
AAGAGCA18500.011.6894852
CTCTCAT6100.00606299.1657371
CCACTAA4650.08.99930730-34
AGTCACC5600.08.9667920-24
CAGTCAC7100.08.25113220-24
CTCGTAT5500.07.86218835-39
ACTAATC4309.094947E-117.785446630-34
ACCACTA5950.07.50194330-34
CACTAAT5302.3646862E-117.10605630-34
CAACGAT9850.0049365727.09530351
CTAATCT5352.1827873E-106.778917330-34
CTTCTGA14406.868461E-46.30940061
Produced by FastQC (version 0.11.5)