FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060867
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAG12004.5438355E-910.2821231
CACGTTT5850.00324488139.887053
CCCAGAA9853.958155E-58.84219551
CTCAAGC8500.007829027.657362
CTCCAAA9600.00265581587.56038431
TTCCACT9600.00275093857.53328232
CTCAAAA15451.4288054E-46.5768011
GTCCTTT12250.00386959066.517361
CTTCCAC13850.00200183766.2884931
CAAAACC12800.00611653926.23731761
CTTAAAA14250.00275543176.11197421
CTTCTGG14850.00436591735.86502551
CTTTATT17708.2355307E-45.7407671
CAAATAT20308.395267E-45.34229473
CTTTCTT21803.5918655E-45.326951