FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1332225
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6705.178945E-611.9973471
CCCACGG8053.32962E-610.8931291
CCCTCTT8406.310067E-59.5693111
CCCCAAT6150.00449254979.5056581
CTCAGGA8050.00437851348.1698471
CTCAAAT14004.261321E-67.8294371
TTACTTG8300.00647321347.82203534
CCCAGTT8700.0087858077.55945631
CTTCCGA13151.3139479E-47.22411871
CTTTCAT23802.3519533E-97.0618461
GTTTGAT17151.0024243E-56.8174691
CCTTCAG12300.00376257426.535141
CTCCAAA13600.00151095346.44777151
CCCGGAT13850.0018554056.33138561
CTCCATT14200.0024560046.17533061
CTTTTAA25203.6407982E-76.0895621
CTTGATT17155.047211E-45.9652861
CTTTTAC20852.7117692E-55.95813271
CTTCAAA24601.5263922E-65.9410361
TTCCAAC15850.00148926195.91656266
Produced by FastQC (version 0.11.5)