FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265774
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCAG7800.00366230388.3332331
CTCTACC9200.00183620237.8501471
CATGAAC12709.8795615E-57.3927371
CCCCGAA10150.004803117.1154041
CTTACAA10250.00528326727.04598571
CTCCGAA11600.00230074326.8485761
CTAAAAT11700.00251830426.7900411
GTTCGAC10850.0091610426.65634541
CAGGTTT12450.00504122076.35453463
ATAAGAT17159.1241986E-56.2905353
CTTCAAT19701.35728515E-56.23229931
CTTCTGA13150.0085187096.04132941
CTTAAAA16200.00190621955.79554031
CATATAT18300.0013397275.52513071
ACAAGAA20359.3108905E-45.30135973
CAAGAAT18250.0077792595.1445341
AAATTCT22606.8026E-45.0918084
Produced by FastQC (version 0.11.5)