FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650432
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2452.5115896E-622.9398697
CACGTCT3159.840187E-720.0723849
ACACGTC2952.701379E-416.6702868
AGCACAC4152.3215798E-413.5428135
AGAGCAC4202.5727376E-413.3795323
GCACACG4805.3710854E-513.17250256
GAGCACA4500.00651789910.9282994
AAGAGCA7904.939892E-59.7836152
ATTCAAT6200.00652876549.0824061
CAGAGAA7350.0026961168.6190181
GAAGAGC8450.00102027398.3300171
CAAAAAT12800.008392426.0490241
CGTTATA4052.007881E-45.550028330-34
CCGGGGG3950.00446871255.01958295-99
Produced by FastQC (version 0.11.5)