FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494682
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATACT4000.002345560812.5291892
CCCTTCT4654.6386215E-412.4764661
CTCCACA4350.00440488211.5175994
CTTCAGA4650.00656466310.9169071
TTGATCT4850.00989025910.3302183
CAAGGAT5800.00328015519.8722283
CTTCCAT8609.0277026E-48.4324961
GTGGGGC1800.00161894057.951823780-84
CAAAGAA11900.00287298146.703481
CTTTTTT17657.9959125E-45.75225161
Produced by FastQC (version 0.11.5)