FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403421
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGGA5802.193848E-411.3309431
CCCGAAT8608.227462E-59.3399761
CCCATCC8054.3762528E-49.071011
CTCGAAA10700.00720164926.8244521
TAGATTT15903.0537027E-56.80758333
CTTCAAT21851.2967266E-66.3497071
ATTGGAT15107.178236E-46.28663061
CATGGAT15308.4145495E-46.20445251
CGTAAAT13150.00760195176.10827351
CTTAAAT15650.00110490116.06569431
CATTAAT16904.2198945E-46.0491291
CCCCCCC2650.0084438696.0473895130-134
ATTTCAC19300.00265076035.2336866
AGATATA22104.739601E-45.2240954
CTTTAAA25258.805467E-55.20550251
GCATATT23802.4278852E-45.15412954
CTTTTAA27355.6784767E-55.07280061