FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883803
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA8103.7712634E-610.7793241
CTTCGTT6759.181086E-49.7013921
GTGTTCG6200.005378239.2997334
CCCAGCT6750.0099908938.6234591
CTCAAAG8350.0063193947.8424431
CTCAAAA12405.549111E-47.04133221
CTTCAAT13652.1897125E-46.92956541
GTTGTTT11700.00232669526.8407251
CTTTTAT16705.375046E-56.5353691
CTTCTCC15200.005497615.7442451
CAAAAAT22655.951224E-45.13981061
Produced by FastQC (version 0.11.5)