FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967746
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTCAGACGAATCTCGTATGCCGT12410.12823612807492876TruSeq Adapter, Index 3 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4700.052.8131877
CACGTCT5100.047.2803769
ACACGTC6800.036.5032278
AGAGCAC9700.025.5965043
GAGCACA10400.025.2378714
AGCACAC10200.025.032085
GCACACG10150.024.4553646
GAAGAGC12950.018.6828161
AAGAGCA14600.018.4692752
GTCACTC4200.012.52854220-24
AGTCACT4400.011.63584320-24
CTCAGAC5300.09.91954125-29
TCACTCA5850.09.47271525-29
CTCGTAT5650.09.31489335-39
ACTCAGA5500.09.04213825-29
TCGTATG6300.08.80539140-44
CTTTCGT6500.0088031548.7580981
AATGAAC7500.00302669228.5104676
CGAATCT5900.08.4356630-34
CTCCAGT6950.08.163938515-19
Produced by FastQC (version 0.11.5)