FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517733
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1605.6004865E-835.4327779
CACACGT2152.2228925E-523.0725067
ACACGTC2054.2727438E-420.741148
GCACACG2801.6947761E-417.71816
AGCACAC2900.004361270514.6632575
TGACTGG3100.006787458413.7172394
AGAGCAC3200.00837338313.2885753
GAGCACA3250.00927625913.0841374
CCCGTTT6250.0064334859.0980321
ATCCGGC10000.00498007477.08723976