FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145869
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGAC6050.00370340179.7304191
CCCAGCT10608.122248E-69.02473451
CAAAGGG9151.4854455E-48.8464411
CTCGAAA8607.82886E-48.5565461
CTTCAAG17151.4123725E-77.72334341
CCCAGAA10859.292639E-47.4603621
CTTATCT12150.0030744956.66213461
CATGAAT12400.00380577226.52781721
CAACAAG18302.3524777E-56.43377541
CACAGAA12600.00449832586.42420051
CCTTTAT15357.971063E-46.23206421
CTATAAA13250.00758889466.1090511
CCCTTTT13550.0095654395.97379541
CTTTCAA17656.665047E-45.83687351
CTTCAAT18600.00129817095.5387541
CTTCCGG19450.0022800125.29670051
CAAAAAG27655.9799375E-55.05656341
Produced by FastQC (version 0.11.5)