FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870352
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAT4200.003082666512.082461
CTTTTAG4600.006079300311.0318111
CTCTTGT4800.00833980110.5721521
CCCTGAG4900.00971657410.3563941
CTATGAA7800.00353719788.3647791
CTTTCAT12054.903303E-57.82101151
CCCGGAT11550.0021106626.9042631
ATGGCAA13100.00111111396.62471638
AGAAAGC14000.0023448666.2002674
CTTTTCA17155.581651E-45.9179391
CGAAGAA15100.00532102865.76117321
CTTTTCT17050.0033422555.52745961
CAAAAAG22656.265696E-45.12104271
Produced by FastQC (version 0.11.5)