FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2862860
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT18256.5452696E-87.5396961
GATCTTA13750.0019538696.3030175
AAACTAC14500.00354249085.97699935
CATAGAT23053.7319132E-65.96960741
CCCAGAT30401.1354132E-85.9556551
CTTGGAT15750.0085909615.51777271
CCCCAAC15750.0085909615.51777271
CCCAAAG21152.4153387E-45.4786391
CATTGGT18000.0064195525.23038861
ATTCACC19600.0031962475.1564676
CACAAGA22555.9941335E-45.1385021
CCAAATT19750.0034679895.1228522
Produced by FastQC (version 0.11.5)