FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023254
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAT5306.2355593E-613.7720551
CTCAATT8153.0990304E-711.6428791
CTCGCAA4500.004906084411.3542951
CTAAAAC6706.898706E-510.8943121
CTCCAAT8759.126554E-610.0103171
CCCCGTC6000.00369709619.7322521
CCTCGGT6950.00116534749.4521881
CTTCTGA11753.4975164E-79.3181141
CATTTGG7700.00584827839.205406145
CTCAAAT8004.1262276E-49.1239871
CTCCAGT7550.00247683688.701021
ACGATTC7500.0025906728.658023
CCTCGAC9602.7269105E-48.3636551
CTCTTTC15352.2585482E-78.0837931
CTTCAAT18105.7516445E-98.0654021
TCTGGTC8100.00518943378.0166848
CTCCGGT8400.0064814567.820561
CCCAGCT8550.0075946517.6833571
CCTCCAT10759.177292E-47.4689381
CTGCAAG13953.4711717E-57.32535171
Produced by FastQC (version 0.11.5)