FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998495
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG7452.0946706E-49.7615891
CGTTGAC7050.0013730949.2838951
TCAACCT8105.326573E-48.8937213
ATTGGCG7600.00296307778.5305238
CTCATGG7750.00324454168.4453491
CCTCAAT7800.00343868748.3912121
CTTCCGA8050.00457045538.1306151
CAACCAG8550.007848417.6551411
CTTCACG9650.00274031147.5361491
CAACAAT13100.00104350896.6617261
CTTTTAA16956.652591E-56.43573761
CTTTTGT16503.2640496E-46.1705081
CCTTTAT14300.00280216996.10269981
CTTCAAT16050.00156904225.8904041
GTTCTTT20151.1323246E-45.77459761
ACAATAA15150.0059213155.70607043
CAATTTC16500.00244254575.67581134
AAAGGCA17900.00104459925.6343464
CAAAAAG23603.2136682E-55.5467331
CTTTTAT21100.00130006445.1699411
Produced by FastQC (version 0.11.5)