FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439906
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAACCT1950.00673070418.639131
CCCAATT2400.001039732418.1731511
GGTAGAT3050.005149557714.3001841
CCTCTAT3150.006377541513.846211
CCCATAT4100.002517774212.4109321
CTTTTAC7650.00288949888.5520711
CTTCAAA7800.00344417948.3876081
GATTATT7800.00360765058.3446724
CTTCTTT15702.2931019E-56.9451531
CTTTATT10700.00749880966.7937011
CTTTTTT19900.003519095.11405231
Produced by FastQC (version 0.11.5)