FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489541
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACG4300.005356264811.2194361
CTCAAAA6500.0010660089.5426851
AAACTTT7550.00423946148.1970854
CTTCCAT7900.0062438017.8515761
CAAAAAC8800.00187404037.8317491
TTTTCCC9806.479876E-47.71451437
ATTTTGC8050.007540217.68794976
CAAAAAA13804.836892E-46.4924071
CTTCTTT13200.0020798776.2653991
Produced by FastQC (version 0.11.5)