FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934181
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGA2900.003817248414.9618981
CAAACGA6354.3910797E-511.3883211
CTCTGTT7100.00154144719.1667961
AGCTTAT8250.0061126917.87168749
CTATAAT10608.7227713E-47.50447371
ACTTTCC9700.003109857.4392786
CTCAAAA12100.00353254126.5741671
CTTGTCG12950.0073274386.12950473