FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449525
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACGA1950.00693432318.5432458
CTTTAAC3203.9247447E-415.8709431
CTCAACA3000.00467397414.5105771
CATCGCT4250.003343735411.9498851
CTTCTCT6203.317945E-511.7020781
CTCACCG4400.00433333511.5425041
CTCTTCG4400.00433333511.5425041
CCCAGCC4500.005124156411.2860041
CTTTCGG6951.06056905E-410.4392631
CTTCAAA8003.981335E-59.9760221
CTTTCAT8003.981335E-59.9760221
CTTCAAT7452.1389742E-49.7386421
ATTTCAT8851.244425E-48.988766
TCCATTT8558.7544176E-48.4583232
CTTCTTT13200.00116409866.59571651
GTTTTTC12750.0060781116.2392575
TTTTTCC17457.158436E-45.802076
Produced by FastQC (version 0.11.5)