FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622889
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT1750.003680004320.6920641
CTTTAGT2050.00917918317.6639581
CTCAAGA5556.8954796E-714.3539541
CTCAAAG6504.0711893E-612.2560681
CTCAGAT4856.69073E-411.9459341
CTCGCCA4250.003391672611.9283661
CCTCGTT5008.6395297E-411.5875561
GATAACG4850.0092866710.419126
CTTTCAA10558.137067E-810.2969991
TCACCGT6050.00433099369.54652
CTTCCGT7050.00142459589.245391
CTTATAA8659.566961E-59.2097631
GAACTTG6350.0064556839.0947555
CTCAAAA8350.00658350257.80598831
CTCCTGC10306.310728E-47.7344121
CTTCCTT13552.1151418E-46.9482581
CAAAAGA13702.4208226E-46.8721821
AGATATT13953.141481E-46.7273484
CTCCTTT11900.0029168836.69449141
TTTCAAT15309.6236134E-46.1342512
Produced by FastQC (version 0.11.5)