FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730046
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2351.5510814E-624.2479749
CACACGT2654.403906E-621.502927
ACACGTC2858.266083E-619.9939448
AGCACAC4008.439141E-616.0263965
GCACACG3758.7263645E-515.1953986
AGAGCAC4152.064953E-413.7317213
GAGCACA4705.9104984E-412.1239874
AAGAGCA10150.0054625377.02094462
Produced by FastQC (version 0.11.5)