FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2282076
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6152.6782436E-713.42104157
CTCGGGT4650.00970540110.3591721
ACACGTC8601.5070438E-59.5976068
CACGTCT9002.5793239E-59.1710459
AGCACAC11454.9965587E-57.8107755
GAGCACA11756.8926944E-57.611354
CTCAGAT9600.0044732377.16817671
GTACGAA10850.00189424756.97658541
AGAGCAC11450.00334686676.6091173
GCACACG12700.00137988636.50030576
CACGAAT14950.00130180425.98386961
CCCACGC16450.00405982565.4382281