FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762876
Sequences flagged as poor quality0
Sequence length100-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA6303.8788552E-511.5269411
CTTCCAG9101.3712652E-610.3742471
CTTCAGG6400.00656016549.0774661
CTTTAAA21151.465196E-87.21046921
CAATGAT10450.00603512076.94925641
CTTCAAT12757.7896233E-46.8347981
CTTTAAT19851.9923536E-66.5851641
CTTCCTT12700.0056003476.2898981
CTTTTAT25153.9251063E-76.06367541
CTTCAAA17004.875394E-45.98044821
CTTTTGA16250.0018488745.8095781
ATACAAT16750.00265203145.6361581
CTCCTTT15950.009551355.4635531
ACATTTA15950.00997388555.4420674
CAAAAGA24857.442564E-55.26018141
CTTCTTT34308.11222E-75.0812641
Produced by FastQC (version 0.11.5)