FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447870
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAAAC9603.0521676E-48.277362
GTTCTTA9000.0014112868.0627321
CCCAGGA8400.00683514177.77477741
CCAAACT11201.7767557E-47.77477651
CCAAGCG10100.0043722667.18461131
CCTTGAA20456.141272E-87.0967811
CCAATGC13802.591517E-46.8357941
CCAGAGA12550.004995446.36024241
CTCCAAC12600.005206716.33500341
CCTTGGC24101.1997654E-66.0219571
CCTTGGT14850.0043791035.86380431
CTGCAAT21454.6566744E-55.75103951
CCTTGAT25904.3059335E-65.60344271
TTGAAGT17150.00373980455.4758263
CCAAGGC18000.0062654975.24077561
CCAGCAT20200.00435759035.0292281
Produced by FastQC (version 0.11.5)