FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5849175
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT44600.05.8520721
CTTCGAT60050.05.7952241
CTCAAAC45450.05.7426271
CTCAAAT51650.05.6147661
CTCAAAA76400.05.59887361
CCCAGAG22107.446212E-55.57697441
CTTAGAA34152.4132532E-85.51981641
CTCAAGC39506.85759E-105.50637961
CCCCTGA18550.00151804885.4717481
CCCACAT41153.45608E-105.4617761
CTCAATT34553.1348463E-85.4559111
CTTCAAA110950.05.3583191
CTCCGAA46606.002665E-115.2897481
TCGATCC20650.00106853295.24796343
CTCAAAG62200.05.24522541
CTTTTAT132650.05.1376271
CTCCGAT39952.8530849E-85.0813981
CTTCAAG93400.05.04554941
CCCATAT44602.8721843E-95.03928381
CCTCGAT46401.544322E-95.00004631
Produced by FastQC (version 0.11.5)