FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037791
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCAG3002.2707685E-417.055451
CCTCAAT8255.532781E-48.8599751
CTTGTAA8550.007499717.69418861
ATTACGA8750.0092208377.51832151
GTCCTTT9750.00288428927.4969021
CTCAAAG10050.00387826217.27311371
TCGATCT10000.00414771547.2231873
CTCCGAA10900.0085249796.7059441
CTCAAAA12400.00408116966.48421571
CTTTTAT18703.559731E-56.25411
CTTGAAG13500.0098646425.95587251
CTTCATT15350.0058285545.7142511
CTTTCTT23401.5515923E-45.3103051
TGCGGCA20800.00117702125.2087789
Produced by FastQC (version 0.11.5)