FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791258
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGT3302.4943578E-517.5892011
CTTTAAT11601.8189894E-1212.509561
GATCGCT4100.002622045312.3460027
CTTTCAC7051.2278435E-410.29155351
ATCCATT8457.555789E-48.5864441
CTCCTGA7800.00350983078.3717821
CTTTCGA7850.00371827148.31845951
CTTTTAA13552.9556231E-68.0319691
CTTGAAT10255.878907E-47.7864391
CTTTCAT12759.779855E-57.397811
CTCCTTT11100.0013739357.190181
CTTTTAT15201.5028236E-57.1600771
CCTCTTT12150.0035611416.5688061
CTTCCTC12200.00371756786.5418851
CTTTATT15007.1445014E-46.28813841
CTTTTCT17359.4661795E-56.2728051
CTTCTTT22351.3923023E-55.8433921
AAAGATT16600.0025068715.6630114
AAGATTT18300.00131631035.53208833
TTTAATA17450.00451882935.3878442
Produced by FastQC (version 0.11.5)