FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543520
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACCT5902.0728778E-512.2566731
CTTAAAT13302.3830744E-68.1557561
CCCGTCG8300.0064595147.82404237
CTCAAGA9550.00261718367.57218551
CTTCGAT15702.484787E-56.9090161
CTATCAA14254.0002845E-46.59710031
CTTATAT18806.4507567E-66.5390661
CTCAAAG16705.8657108E-56.49530271
CCCAGAT13550.00163154236.4042251
CTTTAAA21501.1619668E-66.3905721
CTTCAAT21306.775461E-66.11107351
CTTCTAT17658.3325454E-45.73598431
ATTCAAT19153.8229642E-45.6643111
CTCCATT15350.00656930365.6532411
CTACAAA15650.0081332685.54487231
ATTTTGG18700.00178022145.40233336
TTAAATA19400.00282265625.20757152
TCAATTC18450.0089187525.0847553