FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592537
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTCG1801.477997E-424.2648981
CTCTAAT2950.004090721714.8057021
CTAAGAA3100.005685889614.0892961
CTCTGAT3700.001151219113.771971
CGAGGCG4150.002977239712.1376818
CTCCAAT4200.002986798512.132451
ACTTCGG4400.004610408611.4480396
CTCAATG5450.001700607610.685461
GTTCAAA6708.5303385E-49.7783931
TCCAATT6000.00415179239.5953572
CCAGATT6500.0080257288.8565043
CTTTGAT9452.3640301E-48.4734561
CTTAAAA8050.00452494538.1385371
CTTTTGT10255.6688476E-47.8121141
CTCCTTT12155.152433E-57.78873351
CTTTTGA10406.622549E-47.6994391
GGTATTT8600.0081867147.618051
CTTTTAT12050.00315155866.64515921
GAAGATT10850.0094627876.63273532
CTTTCAA11000.0096715376.61769961
Produced by FastQC (version 0.11.5)